3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells

3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells

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Ovarian cancer is fairly unique in that ovarian caroline gardner cards sale carcinoma cells can detach and spread directly through peritoneal cavity.It has been unclear, however, how detached cancer cells survive in the peritoneum and form spheroid structure.We have recently reported that there is a strong correlation between Tumor-associated macrophages (TAMs)-associated spheroid and clinical pathology of ovarian cancer, and that TAMs promote spheroid formation and tumor growth at early stages of transcoelomic metastasis in orthotopic mouse models.We have established an in vitro spheroid formation assay using a 3D co-culture system in which mouse GFP+F4/80+CD206+ TAMs isolated from spheroids of ovarian cancer-bearing donor tomatolysM-cre mice were mixed with ID8 pure energy jeans cells (TAM:ID8 at a ratio of 1:10) in medium containing 2% Matrigel and seeded onto the 24-well plate precoated with Matrigel.

As transcoelomic metastasis is also associated with many other cancers such as pancreatic and colon cancers, TAM-mediated spheroid formation assay would provide a useful approach to define the molecular mechanism and therapeutic targets for ovarian cancer and other transcoelomic metastasis cancers.